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vector unmasking reagent  (Vector Laboratories)


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    Vector Laboratories vector unmasking reagent
    Vector Unmasking Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unmasking+reagent/Antigen+Unmasking+Solution%2C+Citric+Acid+Based/bio_rxiv__64898__2026__01__06__697856-95-13-16
    Average 96 stars, based on 2600 article reviews
    vector unmasking reagent - by Bioz Stars, 2026-09
    96/100 stars

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    Immunohistochemistry:

    Article Title: The existence of a local 5-hydroxytryptaminergic system in peripheral arteries
    Article Snippet: Bands on the gel were visualized using a Bio-Rad Fluor-S (Hercules, CA, USA). .. Immunohistochemistry Paraffin-embedded tissue sections were dewaxed, unmasked using Unmasking Reagent (Vector Laboratories, Burlingame, CA, USA) and taken through a standard protocol. .. Primary antibody used was anti-TPH antibody (WH-3 monoclonal antibody, 1:500, Sigma), MAO A (H-70, 5 μg mL −1 ; Santa Cruz Biotechnology) in 1.5% blocking serum in phosphate-buffered saline or 1.5% blocking serum as a control.

    Article Title: L‐cysteine/cystathionine‐β‐synthase‐induced relaxation in mouse aorta involves a L‐serine/sphingosine‐1‐phosphate/NO pathway
    Article Snippet: .. For immunohistochemistry, deparaffinized and rehydrated slides were unmasked using unmasking reagent (Vector Laboratories, Burlingame, CA) and treated with 0.3% H 2 O 2 for 12 min before the incubation in serum‐free Protein Block (DakoCytomation, Denmark) for 30 min. .. Sections were then incubated with polyclonal antibody to CBS (RRID:AB_2070973; sc‐67154, Santa Cruz Biotechnology, Dallas, TX; 1:50 dilution), in 1% blocking reagent/0.3% Triton X‐100 (MP Biomedicals, Eschwege, Germany), overnight at 4°C.

    Incubation:

    Article Title: L‐cysteine/cystathionine‐β‐synthase‐induced relaxation in mouse aorta involves a L‐serine/sphingosine‐1‐phosphate/NO pathway
    Article Snippet: .. For immunohistochemistry, deparaffinized and rehydrated slides were unmasked using unmasking reagent (Vector Laboratories, Burlingame, CA) and treated with 0.3% H 2 O 2 for 12 min before the incubation in serum‐free Protein Block (DakoCytomation, Denmark) for 30 min. .. Sections were then incubated with polyclonal antibody to CBS (RRID:AB_2070973; sc‐67154, Santa Cruz Biotechnology, Dallas, TX; 1:50 dilution), in 1% blocking reagent/0.3% Triton X‐100 (MP Biomedicals, Eschwege, Germany), overnight at 4°C.

    Blocking Assay:

    Article Title: L‐cysteine/cystathionine‐β‐synthase‐induced relaxation in mouse aorta involves a L‐serine/sphingosine‐1‐phosphate/NO pathway
    Article Snippet: .. For immunohistochemistry, deparaffinized and rehydrated slides were unmasked using unmasking reagent (Vector Laboratories, Burlingame, CA) and treated with 0.3% H 2 O 2 for 12 min before the incubation in serum‐free Protein Block (DakoCytomation, Denmark) for 30 min. .. Sections were then incubated with polyclonal antibody to CBS (RRID:AB_2070973; sc‐67154, Santa Cruz Biotechnology, Dallas, TX; 1:50 dilution), in 1% blocking reagent/0.3% Triton X‐100 (MP Biomedicals, Eschwege, Germany), overnight at 4°C.

    Staining:

    Article Title: Evidence that fibroblasts derive from epithelium during tissue fibrosis
    Article Snippet: .. PBS–Tween 20 was added wherever PBS was used, and in staining reactions for FSP1, an unmasking reagent (Vector Laboratories Inc., Burlingame, California, USA) was used in the prestain. .. Confocal microscopy was performed using either a Zeiss LSM 410 Laser-Scanning Confocal Microscope with 488 nm excitation for FITC and 543 nm or 568 nm excitation for Texas Red (Vanderbilt Cell Imaging Core), or an Olympus Provis microscope (Olympus America, Melville, New York, USA) with an Optronics MagnaFire imaging system (Optronics, Goleta, California, USA) using U-M41001-FITC and U-M41002TRITC filters, at the Vanderbilt Cardiovascular Histology and Imaging Core.



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